NOD-LtJ Type 1 Diabetes In Mice And The Effect Of Stem Cells – Berashis – Derived From Human Umbilical Cord Blood

NOD-LtJ Type 1 Diabetes In Mice And The Effect Of Stem Cells (Berashis) Derived From Human Umbilical Cord Blood

Norman Ende, Ruifeng Chen, and Richard Mack

Department of Pathology and Laboratory Medicine
New Jersey Medical School
185 South Orange Avenue, MSB-C501
Newark, NJ 07103

(Printed in Journal Of Medicine, Volumes 1-4, 2002)

Key Words: Berashis cell, cord blood, diabetes, NOD/LtJ mice, stem cell

[There is evidence that very immature pleuropotential stem cells – Berashis cells – exist in human umbilical cord blood in small numbers. We feel that these Berashis cells may have similar physiological properties to those attributed to embryonic stem cells. By administering megadoses of human umbilical cord blood mononuclear cells – thus increasing the number of Berashis cells administered – we were able to ameliorate disease and improve the lifespan of NOD/LtJ type 1 diabetic mice.]

Previously we have successfully delayed the onset of vasculitis and death in MRL Lpr/Lpr mice that are considered to have an autoimmune disease similar to human lupus erythematosus.

Likewise, with the use of megadose human umbilical cord blood mononuclear cells, we were able to delay the onset of symptoms and death in SOD1 mice that carry a transgene for amyotrophic lateral sclerosis, considered by some to be an autoimmune disease.

A similar approach was utilized with NOD/LtJ type 1 diabetic mice. By administering megadoses of human umbilical cord blood mononuclear cells, we were able to ameliorate the disease, and improved the life span. This occurred to a greater extent than with bone marrow obtained from congenic mice.

No immunosuppression was utilized in this study.

This study raises the possibility of utilizing human cord blood mononuclear cells in conjunction with pancreatic islet transplantation.

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Commentary – Berashis Cells In Human Umbilical Cord Blood Vs Embryonic Stem Cells

Commentary – Berashis Cells In Human Umbilical Cord Blood Vs Embryonic Stem Cells

Norman Ende
Professor of Pathology and Laboratory Medicine
UMDNJ-New Jersey Medical School
185 South Orange Avenue
Newark, NJ 07103

(Printed in Journal Of Medicine, Volumes 1-4, 2002)

Key Words: Berashis cells, cord blood, embryonic stem cell, human cord blood, therapeutic potential

[Since 1990 we have been aware that human umbilical cord blood had unique cells that displayed reparative ability for various organs in subject mice. We call these cells “Berashis Cells” – beginning cells – to distinguish them from embryonic stem cells, fetal stem cells, adult stem cells, or organ specific stem cells. While much public attention has been given to embryonic stem cell research, this article demonstrates¬†that Berashis Cells from umbilical cord blood have the potential to attain similar ¬†therapeutic¬† results.]

Over the past 3 years there have been numerous articles in medical journals, biological journals, and the lay press concerning the great potential of embryonic stem cells (Weissman, 2002). This has provoked extensive controversies, ethical, political, patient rights, attracting numerous television and press reports.

Throughout these articles, the potential for some of the cells found in cord blood collected from normal infants has been essentially ignored. Even though these human cells have been demonstrated to be therapeutically effective both in non-transgenic animal models and in transgenic models, including such diverse diseases as a mouse model for human lupus erythematosus to transgenic mice for Huntington disease, little attention has been paid to this subject.

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