Effect of Human Umbilical Cord Blood Mononuclear Cells On Preclinical Prostate Cancer Mice “Tramp” Summary: Prostate cancer is almost exclusively a malignancy of men, particularly older men. There is significant evidence that the human immune system deteriorates with age. This deterioration may have a significant role in the development of cancer in the prostate. A […]
NOD-LtJ Type 1 Diabetes In Mice And The Effect Of Stem Cells – Berashis – Derived From Human Umbilical Cord Blood
By administering megadoses of human umbilical cord blood mononuclear cells – thus increasing the number of “Berashis Cells” administered – we were able to ameliorate disease and improve the lifespan of NOD/LtJ type 1 diabetic mice.
While much public attention has been given to embryonic stem cell research, this article demonstrates that Berashis Cells from umbilical cord blood have the potential to attain similar therapeutic results.
This article is an attempt to define very primitive pleuripotential and probably totipotential primitive cells found in umbilical cord blood and their clinical significance. To distinguish these cells from embryonic stem cells, we call these cells “Berashis Cells.”
The article by Wagner et all was very encouraging on the potential use of human umbilical cord blood. On page 1879, 1st column 2nd paragraph, there appears a sentence that raises considerable legal, ethical, and medical’ questions, similar to what occurred in our studies approximately 25 years ago.
We have found that human umbilical cord blood (HUCB) will routinely protect mice exposed to lethal levels of irradiation. At the end of 50 days, over 70% of mice injected with HUBC survived 900 cGy of irradiation, which produced 100% deaths in the uninjected control animals.
The article reported a patient with acute lymphoblastic leukemia who obtained a documented temporary graft following administration of a relatively small quantity of umbilical cord blood matched only for ABO typing.
Utilizing human umbilical cord blood, it has been possible to create in irradiated animals a human to mouse xenograft. To facilitate hematopoietic reconstitution, SJL/J mice, which are functionally low in natural killer (NK) cells, were treated with anti-Asialo GM antibodies (anti-NK) and irradiation prior to injection of cord blood mononuclear cells.
Specimens of umbilical cord blood were collected from 13 routine obstetrical deliveries. All specimens grew erythroid and granulocytic-monocytic colonies. The formation of these various hematopoietic colonies from umbilical cord blood was at least equivalent to bone marrow, and in some instances over 5 times more effective.
A series of eight transfusions consisting of 30 to 85 ml of umbilical cord blood was utilized to establish a hematopoietic transplant in a patient with acute lymphoblastic leukemia on conventional therapy.