NOD-LtJ Type 1 Diabetes In Mice And The Effect Of Stem Cells (Berashis) Derived From Human Umbilical Cord Blood
Norman Ende, Ruifeng Chen, and Richard Mack
Department of Pathology and Laboratory Medicine
New Jersey Medical School
185 South Orange Avenue, MSB-C501
Newark, NJ 07103
(Printed in Journal Of Medicine, Volumes 1-4, 2002)
Key Words: Berashis cell, cord blood, diabetes, NOD/LtJ mice, stem cell
[There is evidence that very immature pleuropotential stem cells – Berashis cells – exist in human umbilical cord blood in small numbers. We feel that these Berashis cells may have similar physiological properties to those attributed to embryonic stem cells. By administering megadoses of human umbilical cord blood mononuclear cells – thus increasing the number of Berashis cells administered – we were able to ameliorate disease and improve the lifespan of NOD/LtJ type 1 diabetic mice.]
Previously we have successfully delayed the onset of vasculitis and death in MRL Lpr/Lpr mice that are considered to have an autoimmune disease similar to human lupus erythematosus.
Likewise, with the use of megadose human umbilical cord blood mononuclear cells, we were able to delay the onset of symptoms and death in SOD1 mice that carry a transgene for amyotrophic lateral sclerosis, considered by some to be an autoimmune disease.
A similar approach was utilized with NOD/LtJ type 1 diabetic mice. By administering megadoses of human umbilical cord blood mononuclear cells, we were able to ameliorate the disease, and improved the life span. This occurred to a greater extent than with bone marrow obtained from congenic mice.
No immunosuppression was utilized in this study.
This study raises the possibility of utilizing human cord blood mononuclear cells in conjunction with pancreatic islet transplantation.