Production Of Human To Mouse Xenografts
By Umbilical Cord Blood
Norman Ende, Dennis Giuliani
Milton Ende, Nicholas M. Ponzio
Department of Pathology
University of Medicine and Dentistry of New Jersey
New Jersey Medical School, Newark, NJ 07103
(Received in final form March 2, 1990)
Utilizing human umbilical cord blood, it has been possible to create in irradiated animals a human to mouse xenograft. To facilitate hematopoietic reconstitution, SJL/J mice, which are functionally low in natural killer (NK) cells, were treated with anti-Asialo GM antibodies (anti-NK) and irradiation prior to injection of cord blood mononuclear cells.
In contrast, SJL/J mice with the “beige” (bg/bg) mutation, which confers a functional NK cell deficiency, required only irradiation for successful transplantation. Human cells, detected by means of DNA probes, were demonstrated in the lungs and lymph nodes of irradiated animals up to 6 months after injection of the human cord blood cells.